Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood
Name
nihms-989658.pdf
Description
Accepted version
Size
945.69 KB
Format
Adobe PDF
Checksum (MD5)
32be385c1f88536001d185c8aa1a4480
Author(s)
Choi, Kyungyong
Ryu, Hyunryul
Siddle, Katherine J.
Piantadosi, Anne
Freimark, Lisa
Park, Daniel J.
Sabeti, Pardis
Han, Jongyoon
Date Issued
March 14, 2018
Journal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Choi, Kyungyong, et al. “Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood.” Analytical Chemistry 90, no. 7 (April 2018): 4657–62.
Version
Author's final manuscript
Abstract
In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous circulation to separate host cells from viral particles and free nucleic acid. We demonstrate that this device effectively reduces white blood cells, red blood cells, and platelets from both whole blood and plasma samples with excellent recovery of viral nucleic acid. Furthermore, microfluidic separation leads to greater viral genome coverage and depth, highlighting an important application of this device in processing clinical samples for viral genome sequencing.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/acs.analchem.7b05200
