The influence of tethered epidermal growth factor on connective tissue progenitor colony formation
Name
Griffith_Influence tethered.pdf
Size
1.86 MB
Format
Adobe PDF
Checksum (MD5)
83acbb19eedf4bab2cfb30b57028903a
Author(s)
Marcantonio, Nicholas A.
Boehm, Cynthia A.
Rozic, Richard J.
Au, Ada
Wells, Alan
Muschler, George F.
Griffith, Linda G.
Date Issued
June 2009
Journal
Biomaterials
Publisher
Elsevier
Citation
Marcantonio, Nicholas A., Cynthia A. Boehm, Richard J. Rozic, Ada Au, Alan Wells, George F. Muschler, and Linda G. Griffith. “The Influence of Tethered Epidermal Growth Factor on Connective Tissue Progenitor Colony Formation.” Biomaterials 30, no. 27 (September 2009): 4629–4638.
Version
Author's final manuscript
Abstract
Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.biomaterials.2009.05.061
