Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system
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Author(s)
Hoehl, Melanie M.
Weißert, Michael
Dannenberg, Arne
Nesch, Thomas
Paust, Nils
von Stetten, Felix
Zengerle, Roland
Slocum, Alexander H.
Steigert, Juergen
Date Issued
February 2014
Journal
Biomedical Microdevices
Publisher
Springer-Verlag
Citation
Hoehl, Melanie M., Michael Weißert, Arne Dannenberg, Thomas Nesch, Nils Paust, Felix von Stetten, Roland Zengerle, Alexander H. Slocum, and Juergen Steigert. “Centrifugal LabTube Platform for Fully Automated DNA Purification and LAMP Amplification Based on an Integrated, Low-Cost Heating System.” Biomed Microdevices (February 25, 2014).
Version
Author's final manuscript
Abstract
This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA purification platform (LabTube). We demonstrate LabTube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VTEC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1 min. The heating system consists of two parallel SMD thick film resistors and a NTC as heating and temperature sensing elements. They are driven by a 3 V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabTube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabTube and it is deployable for a variety of heating and electrical applications.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Mechanical Engineering
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DOI of Published Version
https://doi.org/10.1007/s10544-014-9841-9
