Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo
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Boyden_Optpgenetic astroctye.pdf
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2.14 MB
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Author(s)
Perea, Gertrudis
Yang, Aimei
Sur, Mriganka
Boyden, Edward
Date Issued
February 2014
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Perea, Gertrudis, Aimei Yang, Edward S. Boyden, and Mriganka Sur. “Optogenetic Astrocyte Activation Modulates Response Selectivity of Visual Cortex Neurons in Vivo.” Nature Communications 5 (February 6, 2014).
Version
Final published version
Abstract
Astrocytes play important roles in synaptic transmission and plasticity. Despite in vitro evidence, their causal contribution to cortical network activity and sensory information processing in vivo remains unresolved. Here we report that selective photostimulation of astrocytes with channelrhodopsin-2 in primary visual cortex enhances both excitatory and inhibitory synaptic transmission, through the activation of type 1a metabotropic glutamate receptors. Photostimulation of astrocytes in vivo increases the spontaneous firing of parvalbumin-positive (PV[superscript +]) inhibitory neurons, while excitatory and somatostatin-positive (SOM[superscript +]) neurons show either an increase or decrease in their activity. Moreover, PV[superscript +] neurons show increased baseline visual responses and reduced orientation selectivity to visual stimuli, whereas excitatory and SOM[superscript +] neurons show either increased or decreased baseline visual responses together with complementary changes in orientation selectivity. Therefore, astrocyte activation, through the dual control of excitatory and inhibitory drive, influences neuronal integrative features critical for sensory information processing.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Picower Institute for Learning and Memory
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
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Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported
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DOI of Published Version
https://doi.org/10.1038/ncomms4262
