Measurement of red blood cell mechanics during morphological changes
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6731.full.pdf
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Author(s)
Best, C. A.
Kuriabova, T.
Henle, M. L.
Levine, A. J.
Park, YongKeun
Badizadegan, Kamran
Dasari, Ramachandra Rao
Feld, Michael S
Popescu, Gabriel
Date Issued
June 20, 2018
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Park, Y. et al. “Measurement of Red Blood Cell Mechanics During Morphological Changes.” Proceedings of the National Academy of Sciences 107, 15 (March 2010): 6731–6736 © 2010 National Academy of Sciences
Version
Final published version
Abstract
The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of the cell's morphology and mechanics. These properties, in turn, influence the ability of RBCs to transport oxygen in circulation. Current mechanical measurements of RBCs rely on external loads. Here we apply a noncontact optical interferometric technique to quantify the thermal fluctuations of RBC membranes with 3 nm accuracy over a broad range of spatial and temporal frequencies. Combining this technique with a new mathematical model describing RBC membrane undulations, we measure the mechanical changes of RBCs as they undergo a transition from the normal discoid shape to the abnormal echinocyte and spherical shapes. These measurements indicate that, coincident with this morphological transition, there is a significant increase in the membrane's shear, area, and bending moduli. This mechanical transition can alter cell circulation and impede oxygen delivery. Keywords: membrane dynamics; microbiology; quantitative phase imaging
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Spectroscopy Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.0909533107
