Interstitial Fluid Flow Intensity Modulates Endothelial Sprouting in Restricted Src-Activated Cell Clusters During Capillary Morphogenesis
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Lauffenburger_Interstitial Fluid Flow.pdf
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Author(s)
Lauffenburger, Douglas A.
Kamm, Roger Dale
Genove, Elsa
Borros, Salvador
Semino, Carlos Eduardo
Hernandez Vera, Rodrigo
Alvarez, Lery
Date Issued
July 2009
Journal
Tissue Engineering. Part A
Publisher
Mary Ann Liebert, Inc.
Citation
Hernández Vera, Rodrigo et al. “Interstitial Fluid Flow Intensity Modulates Endothelial Sprouting in Restricted Src-Activated Cell Clusters During Capillary Morphogenesis.” Tissue Engineering Part A 15.1 (2010): 175-185. © 2009 Mary Ann Liebert, Inc.
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Final published version
Abstract
Development of tissues in vitro with dimensions larger than 150 to 200μm requires the presence of a functional vascular network. Therefore, we have studied capillary morphogenesis under controlled biological and biophysical conditions with the aim of promoting vascular structures in tissue constructs. We and others have previously demonstrated that physiological values of interstitial fluid flow normal to an endothelial monolayer in combination with vascular endothelial growth factor play a critical role during capillary morphogenesis by promoting cell sprouting. In the present work, we studied the effect that a range of interstitial flow velocities (0–50μm/min) has in promoting the amount, length, and branching of developing sprouts during capillary morphogenesis. The number of capillary-like structures developed from human umbilical vein endothelial cell monolayers across the interstitial flow values tested was not significantly affected. Instead, the length and branching degree of the sprouts presented a significant maximum at flow velocities of 10 to 20μm/min. More-over, at these same flow values, the phosphorylation level of Src also showed its peak. We discovered that capillary morphogenesis is restricted to patches of Src-activated cells (phosphorylated Src (pSrc)) at the monolayer, suggesting that the transduction pathway in charge of sensing the mechanical stimulus induced by flow is promoting predetermined mechanically sensitive areas (pSrc) to undergo capillary morphogenesis.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1089/ten.tea.2007.0314
