Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
Name
sciadv.abg6383.pdf
Description
Published version
Size
1.21 MB
Format
Adobe PDF
Checksum (MD5)
4b7d0f2fb0b373360093984fe538fd50
Author(s)
Datta, Rupsa
Sivanand, Sharanya
Lau, Allison N
Florek, Logan V
Barbeau, Anna M
Wyckoff, Jeffrey
Skala, Melissa C
Vander Heiden, Matthew G
Date Issued
2022
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Datta, Rupsa, Sivanand, Sharanya, Lau, Allison N, Florek, Logan V, Barbeau, Anna M et al. 2022. "Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors." Science Advances, 8 (3).
Version
Final published version
Abstract
Access to electron acceptors supports oxidized biomass synthesis and can be limiting for cancer cell proliferation, but how cancer cells overcome this limitation in tumors is incompletely understood. Nontransformed cells in tumors can help cancer cells overcome metabolic limitations, particularly in pancreatic cancer, where pancreatic stellate cells (PSCs) promote cancer cell proliferation and tumor growth. However, whether PSCs affect the redox state of cancer cells is not known. By taking advantage of the endogenous fluorescence properties of reduced nicotinamide adenine dinucleotide and oxidized flavin adenine dinucleotide cofactors we use optical imaging to assess the redox state of pancreatic cancer cells and PSCs and find that direct interactions between PSCs and cancer cells promote a more oxidized state in cancer cells. This suggests that metabolic interaction between cancer cells and PSCs is a mechanism to overcome the redox limitations of cell proliferation in pancreatic cancer.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1126/SCIADV.ABG6383
