Genetic engineering of human ES and iPS cells using TALE nucleases
Name
Jaensich_Genetic engineering.pdf
Size
1.27 MB
Format
Adobe PDF
Checksum (MD5)
b14017f044db9431c4c1d79942c82407
Author(s)
Hockemeyer, Dirk
Wang, Haoyi
Kiani, Samira
Lai, Christine S.
Gao, Qing
Cassady, John P.
Cost, Gregory J.
Santiago, Yolanda
Miller, Jeffrey C.
Zeitler, Bryan
Date Issued
July 2011
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Hockemeyer, Dirk et al. “Genetic Engineering of Human Pluripotent Cells Using TALE Nucleases.” Nature Biotechnology 29.8 (2011): 731–734.
Version
Author's final manuscript
Abstract
Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nbt.1927
