teashirt is required for head-versus-tail regeneration polarity in planarians
Name
986239543-MIT.pdf
Description
Full printable version
Size
5.59 MB
Format
Adobe PDF
Checksum (MD5)
b94ada716c1be4bf8d3b64d879298548
Author(s)
Owen, Jared H. (Jared Huntsman)
Advisor(s)
Peter Reddien.
Date Issued
2017
Publisher
Massachusetts Institute of Technology
Abstract
Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes [beta]-catenin-1, wntl, Dvl-1/2, or wntless. teashirt was required for [beta]-catenin-I-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2017.
Cataloged from PDF version of thesis. "t" at beginning of title is letter "t" in lower case.
Includes bibliographical references (pages 28-33).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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