Searching for the nik Operon: How a Ligand-Responsive Transcription Factor Hunts for Its DNA Binding Site
Name
Stultz-2010-Searching for the nik Operon.pdf
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3.64 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Phillips, Christine M.
Stultz, Collin M
Drennan, Catherine L
Date Issued
August 2010
Journal
Biochemistry
Publisher
American Chemical Society (ACS)
Citation
Phillips, Christine M., Collin M. Stultz, and Catherine L. Drennan. “Searching for the Nik Operon: How a Ligand-Responsive Transcription Factor Hunts for Its DNA Binding Site.” Biochemistry 49.36 (2010): 7757–7763. © 2010 American Chemical Society
Version
Final published version
Abstract
Transcription factors regulate a wide variety of genes in the cell and play a crucial role in maintaining cellular homeostasis. A major unresolved issue is how transcription factors find their specific DNA binding sequence in the vast expanse of the cell and how they do so at rates that appear faster than the diffusion limit. Here, we relate an atomic-detail model that has been developed to describe the transcription factor NikR’s mechanism of DNA binding to the broader theories of how transcription factors find their binding sites on DNA. NikR is the nickel regulatory transcription factor for many bacteria, and NikR from Escherichia coli is one of the best studied ligand-mediated transcription factors. For the E. coli NikR protein, there is a wide variety of structural, biochemical, and computational studies that provide significant insight into the NikR−DNA binding mechanism. We find that the two models, the atomic-level model for E. coli NikR and the cellular model for transcription factors in general, are in agreement, and the details laid out by the NikR system may lend additional credence to the current models for transcription factors searching for DNA.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/bi100947k
