Chronically implantable LED arrays for behavioral optogenetics in primates
Name
2020.09.10.291583.full.pdf
Description
Submitted version
Size
2.64 MB
Format
Adobe PDF
Checksum (MD5)
3000afb58ca08fdc9bdfce39279a067b
Author(s)
Rajalingham, Rishi
Sorenson, Michael
Azadi, Reza
Bohn, Simon
DiCarlo, James J
Afraz, Arash
Date Issued
September 2021
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Rajalingham, Rishi, Sorenson, Michael, Azadi, Reza, Bohn, Simon, DiCarlo, James J et al. 2021. "Chronically implantable LED arrays for behavioral optogenetics in primates." Nature Methods, 18 (9).
Version
Original manuscript
Abstract
Optogenetic methods have been widely used in rodent brains, but remain relatively under-developed for nonhuman primates such as rhesus macaques, an animal model with a large brain expressing sophisticated sensory, motor and cognitive behaviors. To address challenges in behavioral optogenetics in large brains, we developed Opto-Array, a chronically implantable array of light-emitting diodes for high-throughput optogenetic perturbation. We demonstrated that optogenetic silencing in the macaque primary visual cortex with the help of the Opto-Array results in reliable retinotopic visual deficits in a luminance discrimination task. We separately confirmed that Opto-Array illumination results in local neural silencing, and that behavioral effects are not due to tissue heating. These results demonstrate the effectiveness of the Opto-Array for behavioral optogenetic applications in large brains.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Center for Brains, Minds, and Machines
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41592-021-01238-9
