Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis
Name
Niles-2011-Combined confocal Raman.pdf
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1010.35 KB
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Checksum (MD5)
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Author(s)
Kang, Jeon Woong
Lue, Niyom
Kong, Chae-Ryon
Barman, Ishan
Dingari, Narahara Chari
Dasari, Ramachandra Rao
Feld, Michael S.
Goldfless, Stephen Jacob
Niles, Jacquin
Date Issued
August 2011
Journal
Biomedical Optics Express
Publisher
Optical Society of America
Citation
Kang, Jeon Woong et al. “Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis.” Biomedical Optics Express 2 (2011): 2484. Web. 19 Oct. 2011. © 2011 Optical Society of America
Version
Final published version
Abstract
We have developed a novel multimodal microscopy system that incorporates confocal Raman, confocal reflectance, and quantitative phase microscopy (QPM) into a single imaging entity. Confocal Raman microscopy provides detailed chemical information from the sample, while confocal reflectance and quantitative phase microscopy show detailed morphology. Combining these intrinsic contrast imaging modalities makes it possible to obtain quantitative morphological and chemical information without exogenous staining. For validation and characterization, we have used this multi-modal system to investigate healthy and diseased blood samples. We first show that the thickness of a healthy red blood cell (RBC) shows good correlation with its hemoglobin distribution. Further, in malaria infected RBCs, we successfully image the distribution of hemozoin (malaria pigment) inside the cell. Our observations lead us to propose morphological screening by QPM and subsequent chemical imaging by Raman for investigating blood disorders. This new approach allows monitoring cell development and cell-drug interactions with minimal perturbation of the biological system of interest.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1364/BOE.2.002484
