[superscript 2]H-DNP-enhanced [superscript 2]H-[superscript 13]C solid-state NMR correlation spectroscopy
Name
2HDNP_100422_final_RGG.pdf
Size
1.1 MB
Format
Adobe PDF
Checksum (MD5)
ede5729aa62ff7a56d7cca27040fc296
Author(s)
Maly, Thorsten
Andreas, Loren
Griffin, Robert Guy
Smith, Albert A.
Date Issued
May 2010
Journal
Physical Chemistry Chemical Physics
Publisher
Royal Society of Chemistry, The
Citation
Maly, Thorsten et al. “2H-DNP-enhanced 2H–13C Solid-state NMR Correlation Spectroscopy.” Physical Chemistry Chemical Physics 12.22 (2010): 5872.
Version
Author's final manuscript
Abstract
Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution [superscript 2]H–[superscript 13]C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved [superscript 2]H–[superscript 13]C correlation spectra with a signal enhancement of ε ≥ 700 compared to a spectrum recorded with microwaves off and otherwise identical conditions. To our knowledge, this is the first time that [superscript 2]2H-DNP has been employed to enhance MAS-NMR spectra of a biologically relevant system. The DNP process is studied using several polarizing agents and the technique is applied to obtain [superscript 2]H–[superscript 13]C correlation spectra of U-[[superscript 2]H, [superscript 13]C] proline.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/c003705b
