Programmable CRISPR-responsive smart materials
Name
aaw5122_ArticleContent_v15.pdf
Description
Accepted version
Size
9.61 MB
Format
Adobe PDF
Checksum (MD5)
84a90563eb42ac20c123ea22ac99fa6e
Author(s)
English, Max A.
Soenksen Martinez, Luis Ruben
Gayet, Raphael V.
de Puig Guixe, Helena
Angenent-Mari, Nicolaas M.
Mao, Angelo S.
Nguyen, Peter Q.
Collins, James J.
Date Issued
August 2019
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
English, Max A. et al. " Programmable CRISPR-responsive smart materials." Science 365, 6455 (August 2019): 780-785 © 2017 The Authors
Version
Author's final manuscript
Abstract
Stimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA–defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black–DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Synthetic Biology Center
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/science.aaw5122
