Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage
Name
Towards microfluidic.pdf
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1.38 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Huang, Sha
Hou, Han Wei
Kanias, Tamir
Sertorio, Jonas Tadeu
Chen, Huichao
Sinchar, Derek
Gladwin, Mark T.
Han, Jongyoon
Date Issued
November 2014
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry, The
Citation
Huang, Sha et al. “Towards Microfluidic-Based Depletion of Stiff and Fragile Human Red Cells That Accumulate During Blood Storage.” Lab Chip 15, 2 (January 2015): 448–458 © 2015 The Royal Society of Chemistry
Version
Author's final manuscript
Abstract
In this study, the effects of prolonged storage on several biophysical properties of red blood cells (RBCs) were investigated. Single cell deformability was used as an important criterion in determining subgroups of RBCs evolved during storage lesion. A deformability-based microfluidic cell sorting technology was applied, which demonstrates the ability to enrich and separate the less deformable subpopulations of stored blood. These less deformable RBC subpopulations were then associated with other important markers such as osmotic fragility indicating cell integrity as well as microparticle content. This work demonstrates a systematic methodology to both monitor and improve banked blood quality, thereby reducing risks related to blood transfusion.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1039/c4lc00768a
