Remotely Activated Protein-Producing Nanoparticles
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Author(s)
Schroeder, Avi
Goldberg, Michael Solomon
Kastrup, Christian
Wang, Yingxia
Jiang, Shan
Joseph, Brian J.
Levins, Christopher G.
Kannan, Sneha T.
Langer, Robert
Anderson, Daniel Griffith
Date Issued
March 2012
Journal
Nano Letters
Publisher
American Chemical Society (ACS)
Citation
Schroeder, Avi, Michael S. Goldberg, Christian Kastrup, Yingxia Wang, Shan Jiang, Brian J. Joseph, Christopher G. Levins, Sneha T. Kannan, Robert Langer, and Daniel G. Anderson. “Remotely Activated Protein-Producing Nanoparticles.” Nano Lett. 12, no. 6 (June 13, 2012): 2685–2689.
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Author's final manuscript
Abstract
The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1021/nl2036047
