Review: Advanced Atomic Force Microscopy Modes for Biomedical Research
Name
biosensors-12-01116-v3.pdf
Size
9.83 MB
Format
Adobe PDF
Checksum (MD5)
4645015e3897152f883c899d5619e540
Author(s)
Xia, Fangzhou
Youcef-Toumi, Kamal
Date Issued
December 2, 2022
Publisher
Multidisciplinary Digital Publishing Institute
Citation
Biosensors 12 (12): 1116 (2022)
Version
Final published version
Abstract
Visualization of biomedical samples in their native environments at the microscopic scale is crucial for studying fundamental principles and discovering biomedical systems with complex interaction. The study of dynamic biological processes requires a microscope system with multiple modalities, high spatial/temporal resolution, large imaging ranges, versatile imaging environments and ideally in-situ manipulation capabilities. Recent development of new Atomic Force Microscopy (AFM) capabilities has made it such a powerful tool for biological and biomedical research. This review introduces novel AFM functionalities including high-speed imaging for dynamic process visualization, mechanobiology with force spectroscopy, molecular species characterization, and AFM nano-manipulation. These capabilities enable many new possibilities for novel scientific research and allow scientists to observe and explore processes at the nanoscale like never before. Selected application examples from recent studies are provided to demonstrate the effectiveness of these AFM techniques.
MIT Department
Massachusetts Institute of Technology. Mechatronics Research Laboratory
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/bios12121116
