Designing Biological Circuits: Synthetic Biology Within the Operon Model and Beyond
Name
BI90CH20_Collins001-024.pdf
Description
Accepted version
Size
715.63 KB
Format
Adobe PDF
Checksum (MD5)
5856e629fec123b6c6f44f3280606b0c
Author(s)
English, Max A.
Gayet, Raphaël V.
Collins, James J.
Date Issued
June 2021
Journal
Annual Review of Biochemistry
Publisher
Annual Reviews
Citation
English, Max A. et al. "Designing Biological Circuits: Synthetic Biology Within the Operon Model and Beyond." Annual Review of Biochemistry 90, 1 (June 2021): 221-244.
Version
Author's final manuscript
Abstract
In 1961, Jacob and Monod proposed the operon model of gene regulation. At the model's core was the modular assembly of regulators, operators, and structural genes. To illustrate the composability of these elements, Jacob and Monod linked phenotypic diversity to the architectures of regulatory circuits. In this review, we examine how the circuit blueprints imagined by Jacob and Monod laid the foundation for the first synthetic gene networks that launched the field of synthetic biology in 2000. We discuss the influences of the operon model and its broader theoretical framework on the first generation of synthetic biological circuits, which were predominantly transcriptional and posttranscriptional circuits. We also describe how recent advances in molecular biology beyond the operon model—namely, programmable DNA- and RNA-binding molecules as well as models of epigenetic and posttranslational regulation—are expanding the synthetic biology toolkit and enabling the design of more complex biological circuits.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1146/annurev-biochem-013118-111914
