Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (V[subscript L]–J[subscript L–C[subscript L) gene clusters
Name
Zimmerman-2012-Immunoglobulin light chain.pdf
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2.64 MB
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Author(s)
Zimmerman, Anastasia M.
Yeo, Gene
Howe, Kerstin
Maddox, Benjamin J.
Steiner, Lisa A.
Alternative Title
Immunoglobulin light chain (IgL) genes in zebrafish: Genomic configurations and inversional rearrangements between (VL–JL–CL) gene clusters
Date Issued
September 2007
Journal
Developmental and Comparative Immunology
Publisher
Elsevier
Citation
Zimmerman, Anastasia M. et al. “Immunoglobulin Light Chain (IgL) Genes in Zebrafish: Genomic Configurations and Inversional Rearrangements Between (VL–JL–CL) Gene Clusters.” Developmental & Comparative Immunology 32.4 (2008): 421–434. Web. 25 May 2012. © 2007 Elsevier Inc.
Version
Final published version
Abstract
In mammals, Immunoglobulin light chain (IgL) are localized to two chromosomal regions (designated κ and λ). Here we report a genome-wide survey of IgL genes in the zebrafish revealing (V[subscript L]–J[subscript L]–C[subscript L]) clusters spanning 5 separate chromosomes. To elucidate IgL loci present in the zebrafish genome assembly (Zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (RSS) motifs were applied. RT-PCR with zebrafish cDNA was used to confirm annotations, evaluate VJ-rearrangement possibilities and show that each chromosomal locus is expressed. In contrast to other vertebrates in which IgL exon usage has been studied, inversional rearrangement between (V[subscript L]–J[subscript L]–C[subscript L]) clusters were found. Inter-cluster rearrangements may convey a selective advantage for editing self-reactive receptors and poise zebrafish by virtue of their extensive numbers of V[subscript L], J[subscript L] and C[subscript L] to have greater potential for immunoglobulin gene shuffling than traditionally studied mice and human models.
Description
Supplementary data associated with this article can be
found in the online version at doi:10.1016/j.dci.2007.
08.005.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.dci.2007.08.005
