Synthesis of proteins by automated flow chemistry
Name
Synthesis_of_Proteins_by_Automated_Flow_Chemistry_v1.pdf
Description
Submitted version
Size
11.74 MB
Format
Adobe PDF
Checksum (MD5)
7bb1bba722e97585c322c31433bcade9
Author(s)
Hartrampf, Nina
Saebi, Azin
Poskus, M.
Gates, Zachary P
Callahan, A. J.
Cowfer, A. E.
Hanna, S.
Antilla, S.
Schissel, Carly K.
Quartararo, Anthony James
Date Issued
May 2020
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Hartrampf, N. et al. "Synthesis of proteins by automated flow chemistry." Science 368, 6494 (February 2020): 980-987 © 2020 American Association for the Advancement of Science
Version
Original manuscript
Abstract
Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids long over 327 consecutive reactions. The machine is rapid: Peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1126/science.abb2491
