Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor
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d1cc04066a.pdf
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Author(s)
Lee, J. Michelle
Kim, Chae Rin
Kim, Sion
Min, Junhong
Lee, Min-Ho
Lee, SangWook
Date Issued
September 2021
Journal
Chemical Communications
Publisher
Royal Society of Chemistry (RSC)
Citation
Lee, J. Michelle et al. "Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor." Forthcoming in Chemical Communications. © 2021 The Royal Society of Chemistry
Version
Final published version
Abstract
We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution Noncommercial 3.0 unported license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/d1cc04066a
