Composability of regulatory sequences controlling transcription and translation in Escherichia coli
Name
Kosuri-2013-Composability of reg.pdf
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Author(s)
Kosuri, Sriram
Goodman, Daniel Bryan
Cambray, Guillaume
Mutalik, Vivek K.
Gao, Yuan
Arkin, Adam P.
Endy, Drew
Church, George M.
Date Issued
August 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Kosuri, S., D. B. Goodman, G. Cambray, V. K. Mutalik, Y. Gao, A. P. Arkin, D. Endy, and G. M. Church. “Composability of Regulatory Sequences Controlling Transcription and Translation in Escherichia Coli.” Proceedings of the National Academy of Sciences 110, no. 34 (August 7, 2013): 14024–14029.
Version
Final published version
Abstract
The inability to predict heterologous gene expression levels precisely hinders our ability to engineer biological systems. Using well-characterized regulatory elements offers a potential solution only if such elements behave predictably when combined. We synthesized 12,563 combinations of common promoters and ribosome binding sites and simultaneously measured DNA, RNA, and protein levels from the entire library. Using a simple model, we found that RNA and protein expression were within twofold of expected levels 80% and 64% of the time, respectively. The large dataset allowed quantitation of global effects, such as translation rate on mRNA stability and mRNA secondary structure on translation rate. However, the worst 5% of constructs deviated from prediction by 13-fold on average, which could hinder large-scale genetic engineering projects. The ease and scale this of approach indicates that rather than relying on prediction or standardization, we can screen synthetic libraries for desired behavior.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1301301110
