Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine
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Sauer_Identification of the cdc48.pdf
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2.64 MB
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3a905f7fa3235350df5733ce7a3d02c4
Author(s)
Barthelme, Dominik
Sauer, Robert T
Date Issued
July 2012
Journal
Science
Publisher
American Association for the Advancement of Science
Citation
Barthelme, D., and R. T. Sauer. “Identification of the Cdc48•20S Proteasome as an Ancient AAA+ Proteolytic Machine.” Science 337, no. 6096 (August 16, 2012): 843-846.
Version
Author's final manuscript
Abstract
Proteasomes are the major energy-dependent proteolytic machines in the eukaryotic and archaeal domains of life. To execute protein degradation, the 20S core peptidase combines with the AAA+ ring of the 19S regulatory particle in eukarya or with the AAA+ proteasome-activating nucleotidase ring in some archaea. Here, we find that Cdc48 and 20S from the archaeon Thermoplasma acidophilum interact to form a functional proteasome. Cdc48 is an abundant and essential double-ring AAA+ molecular machine ubiquitously present in archaea, where its function has been uncertain, and in eukarya where Cdc48 participates by largely unknown mechanisms in diverse cellular processes, including multiple proteolytic pathways. Thus, proteolysis in collaboration with the 20S peptidase may represent an ancestral function of the Cdc48 family.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1126/science.1224352
