A cellular and spatial map of the choroid plexus across brain ventricles and ages
Name
nihms-1690857.pdf
Description
Accepted version
Size
3.53 MB
Format
Adobe PDF
Checksum (MD5)
f2d46f5277c95509c282a5d96e8d1240
Author(s)
Dani, Neil
Herbst, Rebecca H
McCabe, Cristin
Green, Gilad S
Kaiser, Karol
Head, Joshua P
Cui, Jin
Shipley, Frederick B
Jang, Ahram
Dionne, Danielle
Date Issued
2021
Journal
Cell
Publisher
Elsevier BV
Citation
Dani, Neil, Herbst, Rebecca H, McCabe, Cristin, Green, Gilad S, Kaiser, Karol et al. 2021. "A cellular and spatial map of the choroid plexus across brain ventricles and ages." Cell, 184 (11).
Version
Author's final manuscript
Abstract
The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.
MIT Department
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.CELL.2021.04.003
