Synthetic Metabolism: Engineering Biology at the Protein and Pathway Scales
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Prather-Synthetic Metabolism.pdf
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555.57 KB
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Adobe PDF
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Author(s)
Martin, Collin H.
Nielsen, David R.
Solomon, Kevin
Prather, Kristala L. Jones
Date Issued
March 2009
Journal
Chemistry and Biology
Publisher
Elsevier B.V.
Citation
Martin, Collin H. et al. “Synthetic Metabolism: Engineering Biology at the Protein and Pathway Scales.” Chemistry & Biology 16.3 (2009): 277-286.
Version
Author's final manuscript
Abstract
Biocatalysis has become a powerful tool for the synthesis of high-value compounds, particularly so in the case of highly functionalized and/or stereoactive products. Nature has supplied thousands of enzymes and assembled them into numerous metabolic pathways. Although these native pathways can be use to produce natural bioproducts, there are many valuable and useful compounds that have no known natural biochemical route. Consequently, there is a need for both unnatural metabolic pathways and novel enzymatic activities upon which these pathways can be built. Here, we review the theoretical and experimental strategies for engineering synthetic metabolic pathways at the protein and pathway scales, and highlight the challenges that this subfield of synthetic biology currently faces.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.chembiol.2009.01.010
