Accelerating the in vitro emulation of Alzheimer’s disease-associated phenotypes using a novel 3D blood-brain barrier neurosphere co-culture model
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fbioe-11-1251195.pdf
Description
Published version
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3.19 MB
Format
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Checksum (MD5)
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Author(s)
Ko, Eunkyung Clare
Spitz, Sarah
Pramotton, Francesca Michela
Barr, Olivia M
Xu, Ciana
Pavlou, Georgios
Zhang, Shun
Tsai, Alice
Maaser-Hecker, Anna
Jorfi, Mehdi
Date Issued
October 8, 2023
Journal
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Citation
Ko EC, Spitz S, Pramotton FM, Barr OM, Xu C, Pavlou G, Zhang S, Tsai A, Maaser-Hecker A, Jorfi M, Choi SH, Tanzi RE and Kamm RD (2023) Accelerating the in vitro emulation of Alzheimer’s disease-associated phenotypes using a novel 3D blood-brain barrier neurosphere co-culture model. Front. Bioeng. Biotechnol. 11:1251195.
Version
Final published version
Abstract
High failure rates in clinical trials for neurodegenerative disorders such as Alzheimer’s disease have been linked to an insufficient predictive validity of current animal-based disease models. This has created an increasing demand for alternative, human-based models capable of emulating key pathological phenotypes in vitro. Here, a three-dimensional Alzheimer’s disease model was developed using a compartmentalized microfluidic device that combines a self-assembled microvascular network of the human blood-brain barrier with neurospheres derived from Alzheimer’s disease-specific neural progenitor cells. To shorten microfluidic co-culture times, neurospheres were pre-differentiated for 21 days to express Alzheimer’s disease-specific pathological phenotypes prior to the introduction into the microfluidic device. In agreement with post-mortem studies and Alzheimer’s disease in vivo models, after 7 days of co-culture with pre-differentiated Alzheimer’s disease-specific neurospheres, the three-dimensional blood-brain barrier network exhibited significant changes in barrier permeability and morphology. Furthermore, vascular networks in co-culture with Alzheimer’s disease-specific microtissues displayed localized β-amyloid deposition. Thus, by interconnecting a microvascular network of the blood-brain barrier with pre-differentiated neurospheres the presented model holds immense potential for replicating key neurovascular phenotypes of neurodegenerative disorders in vitro.
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DOI of Published Version
https://doi.org/10.3389/fbioe.2023.1251195
