CometChip enables parallel analysis of multiple DNA repair activities
Name
nihms-1737214.pdf
Description
Accepted version
Size
1.57 MB
Format
Adobe PDF
Checksum (MD5)
d775f6be01ac488127c44a76e989538d
Author(s)
Ge, Jing
Ngo, Le P
Kaushal, Simran
Tay, Ian J
Thadhani, Elina
Kay, Jennifer E
Mazzucato, Patrizia
Chow, Danielle N
Fessler, Jessica L
Weingeist, David M
Date Issued
October 2021
Journal
DNA Repair
Publisher
Elsevier BV
Citation
Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay, Elina Thadhani, Jennifer E. Kay, Patrizia Mazzucato, Danielle N. Chow, Jessica L. Fessler, David M. Weingeist, Robert W. Sobol, Leona D. Samson, Scott R. Floyd, Bevin P. Engelward, CometChip enables parallel analysis of multiple DNA repair activities, DNA Repair, Volume 106, 2021, 103176
Version
Author's final manuscript
Abstract
DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays are relatively low throughput and are limited to analysis of one protein or one pathway. Here, we have explored the capacity of the CometChip platform for parallel analysis of multiple DNA repair activities. Taking advantage of the versatility of the traditional comet assay and leveraging micropatterning techniques, the CometChip platform offers increased throughput and sensitivity compared to the traditional comet assay. By exposing cells to DNA damaging agents that create substrates of Base Excision Repair, Nucleotide Excision Repair, and Non-Homologous End Joining, we show that the CometChip is an effective method for assessing repair deficiencies in all three pathways. With these applications of the CometChip platform, we expand the utility of the comet assay for precise, high-throughput, parallel analysis of multiple DNA repair activities.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.dnarep.2021.103176
