In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation
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nihms621465.pdf
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Author(s)
Gierut, J. J.
Haigis, K. M.
Jacks, Tyler E.
Date Issued
March 2014
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory Press
Citation
Gierut, J. J. et al. “In Vivo Delivery of Lenti-Cre or Adeno-Cre into Mice Using Intranasal Instillation.” Cold Spring Harbor Protocols 2014, 3 (March 2014): 307-309 © 2014 Cold Spring Harbor Laboratory Press
Version
Author's final manuscript
Abstract
Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1101/PDB.PROT073445
