Carboxylated Nanoparticle Surfaces Enhance Association with Mucoid Pseudomonas aeruginosa Biofilms
Name
deiss-yehiely-et-al-2024-carboxylated-nanoparticle-surfaces-enhance-association-with-mucoid-pseudomonas-aeruginosa.pdf
Description
Published version
Size
3.81 MB
Format
Adobe PDF
Checksum (MD5)
0e0397e3d0f5b61ded09c98d0dda261b
Author(s)
Deiss-Yehiely, Elad
Dzordzorme, Abigail E
Loiselle, Maggie Elizabeth
Yonker, Lael M
Hammond, Paula T
Date Issued
March 14, 2024
Journal
ACS Applied Materials & Interfaces
Publisher
American Chemical Society
Citation
Elad Deiss-Yehiely, Abigail E. Dzordzorme, Maggie Elizabeth Loiselle, Lael M. Yonker, and Paula T. Hammond. ACS Applied Materials & Interfaces 2024 16 (12), 14573-14582.
Version
Final published version
Abstract
Pseudomonas aeruginosa biofilms comprise three main polysaccharides: alginate, psl, and pel, which all imbue tolerance against exogenous antimicrobials. Nanoparticles (NPs) are an exciting new strategy to overcome the biofilm matrix for therapeutic delivery applications; however, zero existing FDA approvals for biofilm-specific NP formulations can be attributed to the complex interplay of physiochemical forces at the biofilm-NP interface. Here, we leverage a set of inducible, polysaccharide-specific, expressing isogenic P. aeruginosa mutants coupled with an assembled layer-by-layer NP (LbL NP) panel to characterize biofilm-NP interactions. When investigating these interactions using confocal microscopy, alginate-layered NPs associated more than dextran-sulfate-layered NPs with biofilms that had increased alginate production, including biofilms produced by mucoid P. aeruginosa isolates from people with cystic fibrosis. These differences were further confirmed in LbL NPs layered with polysaccharide- or hydrocarbon-based polymers with pendent carboxylate or sulfate functional groups. These data suggest carboxylated NP surfaces have enhanced interactions specifically with mucoid biofilms as compared to sulfated surfaces and lay the foundation for their inclusion as a design element for increasing biofilm-NP interactions and efficacious drug delivery.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/acsami.3c18656
