The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis
Name
Zhou-2014-The contractile ring.pdf
Size
6.49 MB
Format
Adobe PDF
Checksum (MD5)
8a827e16ce665f21b0826c7dc1c3f118
Author(s)
Zhou, Zhou
Munteanu, Emilia Laura
He, Jun
Ursell, Tristan
Bathe, Mark
Huang, Kerwyn Casey
Chang, Fred
Date Issued
October 2014
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Zhou, Zhou, Emilia Laura Munteanu, Jun He, Tristan Ursell, Mark Bathe, Kerwyn Casey Huang, and Fred Chang. “The Contractile Ring Coordinates Curvature Dependent Septum Assembly During Fission Yeast Cytokinesis.” Molecular Biology of the Cell (October 29, 2014).
Version
Final published version
Abstract
The functions of the actin-myosin–based contractile ring in cytokinesis remain to be elucidated. Recent findings show that in the fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization of cell wall fibers outside the plasma membrane, not by the contractile ring. Here we show that one function of the ring is to spatially coordinate septum cell wall assembly. We develop an improved method for live-cell imaging of the division apparatus by orienting the rod-shaped cells vertically using microfabricated wells. We observe that the septum hole and ring are circular and centered in wild-type cells and that in the absence of a functional ring, the septum continues to ingress but in a disorganized and asymmetric manner. By manipulating the cleavage furrow into different shapes, we show that the ring promotes local septum growth in a curvature-dependent manner, allowing even a misshapen septum to grow into a more regular shape. This curvature-dependent growth suggests a model in which contractile forces of the ring shape the septum cell wall by stimulating the cell wall machinery in a mechanosensitive manner. Mechanical regulation of the cell wall assembly may have general relevance to the morphogenesis of walled cells.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1091/mbc.E14-10-1441
