BBF RFC 104: BrickClip – rapid assembly of multiple RFC10 BioBricks
Name
BBFRFC104.pdf
Size
575.98 KB
Format
Adobe PDF
Checksum (MD5)
40aed700a1f6f6a6423616098e50e9bf
Author(s)
Trubitsyna, Maryia
Chan, Karen
Cai, Yizhi
Elfick, Alistair
French, Chris
Date Issued
March 17, 2015
Series/Report no.
BBF RFC;104
Abstract
This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific donor and acceptor vectors. BrickClip allows assembly of up to six parts from existing parts collections, including the Registry of Standard Biological Parts, in a single reaction, in any desired order. The resulting product is exactly the same as would be obtained from normal RFC10 BioBrick assembly. In contrast to other commonly used methods such as Gibson assembly, BrickClip does not require ordering new oligonucleotides for each assembly; while assembly oligonucleotides are required, these are part-specific and may be re-used in any assembly involving a given part. BrickClip is a special case of a more general assembly method – PaperClip.
Subjects
assembly
Terms of Use
Attribution-NoDerivs 3.0 United States
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