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Dual modes of CRISPR-associated transposon homing
Name
nihms-1716197.pdf
Description
Accepted version
Size
1.27 MB
Format
Adobe PDF
Checksum (MD5)
ffe12fa4235783199977b81b1d45a0b7
Author(s)
Saito, Makoto
Ladha, Alim
Strecker, Jonathan
Faure, Guilhem
Neumann, Edwin
Altae-Tran, Han
Macrae, Rhiannon K
Zhang, Feng
Date Issued
April 2021
Journal
Cell
Publisher
Elsevier BV
Citation
Saito, Makoto, Ladha, Alim, Strecker, Jonathan, Faure, Guilhem, Neumann, Edwin et al. 2021. "Dual modes of CRISPR-associated transposon homing." Cell, 184 (9).
Version
Author's final manuscript
Abstract
Tn7-like transposons have co-opted CRISPR systems, including class 1 type I-F, I-B, and class 2 type V-K. Intriguingly, although these CRISPR-associated transposases (CASTs) undergo robust CRISPR RNA (crRNA)-guided transposition, they are almost never found in sites targeted by the crRNAs encoded by the cognate CRISPR array. To understand this paradox, we investigated CAST V-K and I-B systems and found two distinct modes of transposition: (1) crRNA-guided transposition and (2) CRISPR array-independent homing. We show distinct CAST systems utilize different molecular mechanisms to target their homing site. Type V-K CAST systems use a short, delocalized crRNA for RNA-guided homing, whereas type I-B CAST systems, which contain two distinct target selector proteins, use TniQ for RNA-guided DNA transposition and TnsD for homing to an attachment site. These observations illuminate a key step in the life cycle of CAST systems and highlight the diversity of molecular mechanisms mediating transposon homing.
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licens
Persistent DSpace Link
DOI of Published Version
10.1016/j.cell.2021.03.006
