Temporal Imaging of Live Cells by High-Speed Confocal Raman Microscopy
Name
materials-14-03732.pdf
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2.32 MB
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Unknown
Checksum (MD5)
65c56d6460ea9778477bf687e12744cb
Author(s)
Kang, Jeon Woong
Nguyen, Freddy T.
Lue, Niyom
Date Issued
July 2021
Publisher
Multidisciplinary Digital Publishing Institute
Citation
Materials 14 (13): 3732 (2021)
Version
Final published version
Abstract
Label-free live cell imaging was performed using a custom-built high-speed confocal Raman microscopy system. For various cell types, cell-intrinsic Raman bands were monitored. The high-resolution temporal Raman images clearly delineated the intracellular distribution of biologically important molecules such as protein, lipid, and DNA. Furthermore, optical phase delay measured using quantitative phase microscopy shows similarity with the image reconstructed from the protein Raman peak. This reported work demonstrates that Raman imaging is a powerful label-free technique for studying various biomedical problems in vitro with minimal sample preparation and external perturbation to the cellular system.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Spectroscopy Laboratory
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/ma14133732
