High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography
Name
Kim-2014-High-resolution thre.pdf
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Author(s)
Kim, Kyoohyun
Yoon, HyeOk
Dao, Ming
Park, YongKeun
Diez Silva, Monica
Dasari, Ramachandra Rao
Date Issued
June 2013
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Kim, Kyoohyun, HyeOk Yoon, Monica Diez-Silva, Ming Dao, Ramachandra R. Dasari, and YongKeun Park. “High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography.” Journal of Biomedical Optics 19, no. 1 (January 1, 2014): 011005. © 2014 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
https://doi.org/10.1117/1.JBO.19.1.011005
