Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing
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Lu_Engineering modular.pdf
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Author(s)
Pires, Diana P.
Ando, Hiroki
Lemire, Sebastien
Lu, Timothy K
Date Issued
September 2015
Journal
Cell Systems
Publisher
Elsevier
Citation
Ando, Hiroki, et al. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing.” Cell Systems 1, 3 (September 2015): 187–196 © 2015 Elsevier Inc
Version
Author's final manuscript
Abstract
Bacteria are central to human health and disease, but existing tools to edit microbial consortia are limited. For example, broad-spectrum antibiotics are unable to precisely manipulate bacterial communities. Bacteriophages can provide highly specific targeting of bacteria, but assembling well-defined phage cocktails solely with natural phages can be a time-, labor- and cost-intensive process. Here, we present a synthetic biology strategy to modulate phage host ranges by engineering phage genomes in Saccharomyces cerevisiae. We used this technology to redirect Escherichia coli phage scaffolds to target pathogenic Yersinia and Klebsiella bacteria, and conversely, Klebsiella phage scaffolds to target E. coli by modular swapping of phage tail components. The synthetic phages achieved efficient killing of their new target bacteria and were used to selectively remove bacteria from multi-species bacterial communities with cocktails based on common viral scaffolds. We envision this approach accelerating phage biology studies and enabling new technologies for bacterial population editing.
MIT Department
MIT Synthetic Biology Center
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.cels.2015.08.013
