An expanded search for RNA-programmable genomic engineering effectors
Name
965549261-MIT.pdf
Description
Full printable version
Size
2.37 MB
Format
Adobe PDF
Checksum (MD5)
e20780f7b353c7a6819cab297ad7bd59
Author(s)
Smargon, Aaron Andrew
Advisor(s)
Feng Zhang.
Alternative Title
Expanded search for ribonucleic acid-programmable genomic engineering effectors
Date Issued
2016
Publisher
Massachusetts Institute of Technology
Abstract
A biocomputational pipeline was designed and implemented to mine through metagenomic datasets for novel Class 2 CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) single effectors, akin to the revolutionary genome-engineering tools Cas9 and Cpf1. Whereas previous search strategies relied on protein proximity to CRISPR-associated spacer acquisition proteins Cas1 and Cas2, this approach was seeded on CRISPR arrays alone. What resulted was the discovery of a potential new Class 2 CRISPR system, with two subtypes as characterized by distinct putative accessory proteins. Follow-up experimental work is required to assess the system's activity: first, in the presence and absence of the accessory protein; and second, as a single effector protein capable of precise genome engineering in prokaryotic and eukaryotic cells.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 31-33).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
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