Control of a neuronal morphology program by an RNA-binding zinc finger protein, Unkempt
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Murn-2015-Control of a neurona.pdf
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Author(s)
Murn, Jernej
Zarnack, Kathi
Yang, Yawei J.
Durak, Omer
Murphy, Elisabeth A.
Cheloufi, Sihem
Gonzalez, Dilenny M.
Teplova, Marianna
Curk, Tomaž
Zuber, Johannes
Date Issued
March 2015
Journal
Genes & Development
Publisher
Cold Spring Harbor Laboratory Press
Citation
Murn, Jernej, Kathi Zarnack, Yawei J. Yang, Omer Durak, Elisabeth A. Murphy, Sihem Cheloufi, Dilenny M. Gonzalez, et al. “Control of a Neuronal Morphology Program by an RNA-Binding Zinc Finger Protein, Unkempt.” Genes Dev. 29, no. 5 (March 1, 2015): 501–512.
Version
Final published version
Abstract
Cellular morphology is an essential determinant of cellular function in all kingdoms of life, yet little is known about how cell shape is controlled. Here we describe a molecular program that controls the early morphology of neurons through a metazoan-specific zinc finger protein, Unkempt. Depletion of Unkempt in mouse embryos disrupts the shape of migrating neurons, while ectopic expression confers neuronal-like morphology to cells of different nonneuronal lineages. We found that Unkempt is a sequence-specific RNA-binding protein and identified its precise binding sites within coding regions of mRNAs linked to protein metabolism and trafficking. RNA binding is required for Unkempt-induced remodeling of cellular shape and is directly coupled to a reduced production of the encoded proteins. These findings link post-transcriptional regulation of gene expression with cellular shape and have general implications for the development and disease of multicellular organisms.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
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DOI of Published Version
https://doi.org/10.1101/gad.258483.115
