Assessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique
Name
Lin-2011-Assessment of liver.pdf
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2.37 MB
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Checksum (MD5)
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Author(s)
Lin, Jian
Lu, Fake
Zheng, Wei
Xu, Shuoyu
Tai, Dean
Yu, Hanry
Huang, Zhiwei
Date Issued
November 2011
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Citation
Lin, Jian et al. “Assessment of Liver Steatosis and Fibrosis in Rats Using Integrated Coherent anti-Stokes Raman Scattering and Multiphoton Imaging Technique.” Journal of Biomedical Optics 16.11 (2011): 116024. Web. 15 Feb. 2012. © 2011 SPIE - International Society for Optical Engineering
Version
Final published version
Abstract
We report the implementation of a unique integrated coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and two-photon excitation fluorescence (TPEF) microscopy imaging technique developed for label-free monitoring of the progression of liver steatosis and fibrosis generated in a bile duct ligation (BDL) rat model. Among the 21 adult rats used in this study, 18 rats were performed with BDL surgery and sacrificed each week from weeks 1 to 6 (n = 3 per week), respectively; whereas 3 rats as control were sacrificed at week 0. Colocalized imaging of the aggregated hepatic fats, collagen fibrils, and hepatocyte morphologies in liver tissue is realized by using the integrated CARS, SHG, and TPEF technique. The results show that there are significant accumulations of hepatic lipid droplets and collagen fibrils associated with severe hepatocyte necrosis in BDL rat liver as compared to a normal liver tissue. The volume of normal hepatocytes keeps decreasing and the fiber collagen content in BDL rat liver follows a growing trend until week 6; whereas the hepatic fat content reaches a maximum in week 4 and then appears to stop growing in week 6, indicating that liver steatosis and fibrosis induced in a BDL rat liver model may develop at different rates. This work demonstrates that the integrated CARS and multiphoton microscopy imaging technique has the potential to provide an effective means for early diagnosis and detection of liver steatosis and fibrosis without labeling.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1117/1.3655353
