Closing gaps in the human genome using sequencing by synthesis
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Gnerre_closing gaps in the human genome.pdf
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Author(s)
Arachchi, Harindra M.
Green, Lisa M.
Zody, Michael C.
Lennon, Niall
Gnerre, Sante
Berlin, Aaron M.
Nusbaum, Chad
Garber, Manuel
Date Issued
June 2009
Journal
Genome Biology
Publisher
BioMed Central Ltd.
Citation
M. Garber, M. Zody, H. Arachchi, A. Berlin, S. Gnerre, L. Green, N. Lennon, and C. Nusbaum, “Closing gaps in the human genome using sequencing by synthesis,” Genome Biology, vol. 10, 2009, p. R60.
Version
Final published version
Abstract
The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15.
MIT Department
Broad Institute of MIT and Harvard
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/gb-2009-10-6-r60
