Regulation of thymocyte positive selection and motility by GIT2
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Irvine_Regulation of thymocyte.pdf
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Author(s)
Phee, Hyewon
Dzhagalov, Ivan
Mollenauer, Marianne
Wang, Yana
Irvine, Darrell J.
Robey, Ellen
Weiss, Arthur
Date Issued
May 2010
Journal
Nature Immunology
Publisher
Nature Publishing Group
Citation
Phee, Hyewon, Ivan Dzhagalov, Marianne Mollenauer, Yana Wang, Darrell J Irvine, Ellen Robey, and Arthur Weiss. “Regulation of thymocyte positive selection and motility by GIT2.” Nature Immunology 11, no. 6 (May 2, 2010): 503-511.
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Author's final manuscript
Abstract
Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein–coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2−/− double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2−/− thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/ni.1868
