No current evidence for widespread dosage compensation in S. cerevisiae
Name
Torres-2016-No current evidence.pdf
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4.04 MB
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Author(s)
Springer, Michael
Torres, Eduardo M.
Amon, Angelika B
Date Issued
March 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Torres, Eduardo M., Michael Springer, and Angelika Amon. "No current evidence for widespread dosage compensation in S. cerevisiae." eLife 2016;5:e10996
Version
Final published version
Abstract
Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.7554/eLife.10996
