Spectrally resolved multidepth fluorescence imaging
Name
Luo-2011-Spectrally resolved.pdf
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1.3 MB
Format
Adobe PDF
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Author(s)
Luo, Yuan
Zervantonakis, Ioannis
Oh, Se Baek
Kamm, Roger Dale
Barbastathis, George
Date Issued
September 2011
Journal
Journal of Biomedical Optics
Publisher
SPIE - International Society for Optical Engineering
Citation
Luo, Yuan et al. “Spectrally Resolved Multidepth Fluorescence Imaging.” Journal of Biomedical Optics 16.9 (2011): 096015. Web. 15 Feb. 2012. © 2011 SPIE - International Society for Optical Engineering
Version
Final published version
Abstract
We present a multicolor fluorescence imaging modality to visualize in real-time tissue structures emitting multispectral fluorescent light from different focal depths. Each designated spectrum of fluorescent emission from a specific depth within a volumetric tissue is probed by a depth-spectrum selective holographic grating. The grating for each fluorescent color are multiplexed within a volume hologram, which enables simultaneously obtaining multicolored fluorescent information at different depths within a biological tissue sample. We demonstrate the imaging modality's ability to obtain laser-induced multicolored fluorescence images of a biological sample from different depths without scanning. We also experimentally demonstrate that the imaging modality can be simultaneously operated at both fluorescent and bright field modes to provide complementary information of volumetric tissue structures at different depths in real-time.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1117/1.3626211
