Automatic Quantifications of Dynamics of Genetic Circuits in a Single Cell in Chemostat
Name
Huang and Del Vecchio_2016.pdf
Size
21.92 MB
Format
Adobe PDF
Checksum (MD5)
c879b751e9b6be23426dc8feee557832
Author(s)
Huang, Hsin-Ho
Del Vecchio, Domitilla
Date Issued
August 31, 2015
Abstract
Modular design of genetic circuits requires iterations of experimental and theoretical efforts to make the design process reliable and the resulting behaviors predictable. This technical report demonstrates a fully automatic experiment procedure by using the MSP FlowCytoPrep 5000 Sample Prep system to deliver samples prepared from multiplex miniature stirred tank reactors (Flexostat) to the flow cytometer BD Accuri C6 to quantify dynamics of an transcription activator cascade in a single cell in chemostat.
Subjects
genetic circuits; chemostat; multiplex miniature stirred tank reactor; FlowCytoPrep 5000; automatic flow cytometry
Terms of Use
Attribution-NonCommercial-ShareAlike 3.0 United States
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