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A steady-state model of microbial acclimation to substrate limitation
Name
pcbi.1008140.pdf
Description
Published version
Size
1.48 MB
Format
Adobe PDF
Checksum (MD5)
0b0a0fd03d73e87b2a49943b6d792684
Author(s)
Casey, John R
Follows, Michael J
Date Issued
2020
Journal
PLoS Computational Biology
Publisher
Public Library of Science (PLoS)
Version
Final published version
Abstract
Microbes acclimate to changes in substrate availability by altering the number of transporters on the cell surface, however there is some disagreement on just how. We revisit the physics of substrate uptake and consider the steady-state scenario whereby cells have acclimated to maximize fitness. Flux balance analysis of a stoichiometric model of Escherichia coli was used in conjunction with quantitative proteomics data and molecular modeling of membrane transporters to reconcile these opposing views. An emergent feature of the proposed model is a critical substrate concentration S*, which delineates two rate limits. At concentrations above S*, transporter abundance can be regulated to maintain uptake rates as demanded by maximal growth rates, whereas below S*, uptake rates are strictly diffusion limited. In certain scenarios, the proposed model can take on a qualitatively different shape from the familiar hyperbolic kinetics curves, instead resembling the long-forgotten Blackman kinetics.
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
10.1371/JOURNAL.PCBI.1008140
