NeuroLINCS Proteomics: Defining human-derived iPSC proteomes and protein signatures of pluripotency
Name
s41597-022-01687-7.pdf
Description
Published version
Size
3.29 MB
Format
Adobe PDF
Checksum (MD5)
2beaf20380de759362d35620fd2af2e6
Author(s)
Fraenkel, Ernest
Date Issued
January 11, 2023
Journal
Scientific Data
Publisher
Springer Science and Business Media LLC
Citation
Fraenkel, Ernest. 2023. "NeuroLINCS Proteomics: Defining human-derived iPSC proteomes and protein signatures of pluripotency." Scientific Data, 10 (1).
Version
Final published version
Abstract
AbstractThe National Institute of Health (NIH) Library of integrated network-based cellular signatures (LINCS) program is premised on the generation of a publicly available data resource of cell-based biochemical responses or “signatures” to genetic or environmental perturbations. NeuroLINCS uses human inducible pluripotent stem cells (hiPSCs), derived from patients and healthy controls, and differentiated into motor neuron cell cultures. This multi-laboratory effort strives to establish i) robust multi-omic workflows for hiPSC and differentiated neuronal cultures, ii) public annotated data sets and iii) relevant and targetable biological pathways of spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). Here, we focus on the proteomics and the quality of the developed workflow of hiPSC lines from 6 individuals, though epigenomics and transcriptomics data are also publicly available. Known and commonly used markers representing 73 proteins were reproducibly quantified with consistent expression levels across all hiPSC lines. Data quality assessments, data levels and metadata of all 6 genetically diverse human iPSCs analysed by DIA-MS are parsable and available as a high-quality resource to the public.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41597-022-01687-7
