Engineering humanized mice for improved hematopoietic reconstitution
Name
Final-2012-CMI-Humice review.pdf
Size
367.49 KB
Format
Adobe PDF
Checksum (MD5)
6ba46f27019ba92cdbf88befc5ec33d7
Author(s)
Drake, Adam
Chen, Jianzhu
Chen, Qingfeng
Date Issued
May 2012
Journal
Cellular and Molecular Immunology
Publisher
Nature Publishing Group
Citation
Drake, Adam C, Qingfeng Chen, and Jianzhu Chen. “Engineering Humanized Mice for Improved Hematopoietic Reconstitution.” Cellular and Molecular Immunology 9.3 (2012): 215–224.
Version
Author's final manuscript
Abstract
Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/cmi.2012.6
