A microfabricated deformability-based flow cytometer with application to malaria
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Han_A microfabricated.pdf
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Author(s)
Bow, Hansen
Pivkin, Igor V.
Diez Silva, Monica
Goldfless, Stephen Jacob
Dao, Ming
Niles, Jacquin
Suresh, Subra
Han, Jongyoon
Date Issued
February 2011
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry
Citation
Bow, Hansen et al. “A Microfabricated Deformability-based Flow Cytometer with Application to Malaria.” Lab on a Chip 11.6 (2011): 1065.
Version
Author's final manuscript
Abstract
Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 10[superscript 3] to 10[superscript 4] individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1039/c0lc00472c
