Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip
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Vaillier-2014-Comprehensive analys.pdf
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Author(s)
Vaillier, Clarisse
Honegger, Thibault
Kermarrec, Frederique
Gidrol, Xavier
Peyrade, David
Date Issued
April 2014
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Vaillier, Clarisse, Thibault Honegger, Frederique Kermarrec, Xavier Gidrol, and David Peyrade. “Comprehensive Analysis of Human Cells Motion Under an Irrotational AC Electric Field in an Electro-Microfluidic Chip.” Edited by Aristides Docoslis. PLoS ONE 9, no. 4 (April 15, 2014): e95231.
Version
Final published version
Abstract
AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0095231
