Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery
Name
Mammalian synthetic.pdf
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525.65 KB
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Author(s)
Endo, Kei
Saito, Hirohide
Wroblewska, Liliana
Kitada, Tasuku
Siciliano, Velia
DiAndreth, Breanna Elizabeth
Weiss, Ron
Date Issued
August 2015
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Wroblewska, Liliana et al. “Mammalian Synthetic Circuits with RNA Binding Proteins for RNA-Only Delivery.” Nature Biotechnology 33.8 (2015): 839–841.
Version
Author's final manuscript
Abstract
Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nbt.3301
