Beyond Single Clones: High-Throughput Sequencing in Antibody Discovery
Name
nihms-2070483.pdf
Description
Accepted version
Size
700.13 KB
Format
Adobe PDF
Checksum (MD5)
944b8bfca94712222208160078c4d1a3
Author(s)
Fahad, Ahmed S
Gutiérrez-Gonzalez, Matías F
Madan, Bharat
DeKosky, Brandon J
Date Issued
2025
Journal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory
Version
Author's final manuscript
Abstract
Antibody repertoire sequencing and display library screening are powerful approaches for antibody discovery and engineering that can connect DNA sequence with antibody function. Antibody display and screening studies have made a tremendous impact on immunology and biotechnology over the last decade, accelerated by technological advances in high-throughput DNA sequencing techniques. Indeed, bioinformatic analysis of antibody DNA library data has now taken a central role in modern antibody drug discovery, and is also critical for many ongoing studies of human immune development. Here, we describe current trends in antibody DNA library screening and analysis, and introduce a selection of protocols describing fundamental bioinformatic techniques to enable scientists to efficiently study antibody DNA libraries.
Terms of Use
Creative Commons Attribution-NonCommercial-ShareAlike
Persistent DSpace Link
DOI of Published Version
https://cshprotocols.cshlp.org/content/2025/1/pdb.top107772.long
